RNA sequencing (RNA-seq) revolutionizes the understanding of cellular functionality by providing deep insights into gene expression and the transcriptome. Utilizing next-generation sequencing (NGS), RNA-seq and converts single-stranded messenger RNAs (mRNAs) into complementary DNA (cDNA) for thorough analysis. Key applications include discovering novel transcripts, studying developmental mechanisms, identifying biomarkers, and enhancingics analyses. This powerful technique is essential for exploring transcript variations and their implications in various biological contexts.

Our Whole Transcriptome Sequencing service offers researchers advanced NGS solutions delivering comprehensive bioinformatic analyses of all RNA transcripts, including mRNA and non-coding RNA (lncRNA, sRNA, and circRNA). Utilizing a paired-end sequencing approach, we accurately quantify gene and transcript levels, uncover splice variants, and reveal novel transcriptome characteristics. This innovative method allows for the exploration of transcriptional and regulatory network mechanisms providing valuable insights into interaction functionalities from a holistic transcriptomic perspective. Key applications include profiling mRNA and ncRNA in a single run, exploring miRNA sponge and target regulatory elements, and investigating regulatory networks among lncRNA/circRNA-miRNA-gene pairs.

Small RNA species generally include the most common and well-studied microRNA (miRNA), small interfering RNA (siRNA), and piwi-interacting RNA (piRNA), as well as other types of small RNA, such as small nucleolar RNA (snoRNA) and small nuclear RNA (snRNA). Small RNA is a type of lowly abundant, short in length (<200 nt), non-protein-coding RNAs that lack polyadenylation. Small RNA populations can vary significantly among different tissue types and species. Generally, small RNAs are formed by fragmentation of longer RNA sequences with the help of dedicated sets of enzymes and other proteins.

Dual RNA Sequencing is an innovative technique that allows researchers to simultaneously analyze the transcriptomes of both host cells and pathogens. This powerful approach provides insights into the interactions between different biological entities, revealing how they influence each other's gene expression. By capturing the complexities of these interactions, Dual RNA Sequencing enhances our understanding of various biological processes, paving the way for advancements in disease research and therapeutic development.

Bacterial RNA sequencing is a powerful technique used to analyze the transcriptome of bacteria, providing insights into gene expression and regulation. This method captures the full range of RNA molecules, including mRNA, rRNA, and non-coding RNAs, enabling researchers to understand bacterial behavior under various. With applications in microbiology, medicine, and biotechnology, bacterial RNA sequencing helps in new biomarkers and understanding pathogenic mechanisms. By leveraging advanced sequencing technologies, scientists can gain a comprehensive view of bacterial function and adaptation.

Degradome sequencing is powerful technique used to analyze RNA degradation patterns in various organisms. By providing insights into gene expression regulation and post-transcriptional modifications, it helps researchers understand the dynamics of RNA molecules within cells. This method is particularly valuable in studying small RNAs and their roles in gene silencing and other cellular processes. With its ability to uncover complex RNA interactions, degradome sequencing is an essential tool for advancing our knowledge in molecular biology.

Ribo-seq is a widely used method in translatome sequencing that captures rib-protected RNA fragments, known as "ribosome footprints," which are actively being translated. This technique involves digesting cellular RNA with RNases to isolate fragments around 30 nucleotides long, which are then sequenced and analyzed. Ribo-seq offers insights into protein translation efficiency, aids in discovering new proteins or peptides, and helps researchers assess gene expression and translation. Our Ribosome Profiling Service enhances understanding of gene regulation by identifying actively translating transcripts and determining protein synthesis rates.

Exosomal RNA sequencing is a comprehensive service that analyzes the unique subsets of microRNAs, mRNAs, long noncoding RNAs (lncRNAs), and other non-coding RNAs found in exosomes. These exosomal components play a crucial role in cell-cell communication and have the potential to serve as biomarkers for disease detection and monitoring. By identifying changes in exosomal miRNA in various disease states, researchers uncover sequence signatures that aid in diagnosis and prognosis. Additionally, profiling exosomal mRNA can provide valuable insights for biomarker discovery from blood, saliva, and urine samples.