Whole Genome Sequencing (WGS) provides a deep insight into the DNA sequence of humans, animals, plants, and microbial genomes, with data analysis at the individual or population level. SNP/INDEL/CNV/SV and other variants of the genome can be fully analysed. Our sequencing analysis enables the identification of somatic and germline mutations as well as customized patterns of cancers and other diseases.

Centyle Biotech provides bisulfite sequencing including Whole Genome Bisulfite Sequencing (WGBS) and Reduced-Representation Bisulfite Sequencing (RRBS) for the detection of methylated cytosines on a genome-wide scale with a single nucleotide resolution. This provides insight into the modifying effects of methylation on gene function and expression. With our ChIP-seq and RIP-seq services, protein-DNA interactions (ChIP-seq) and RNA-protein interactions (RIP-seq) can be effectively analyzed.

Metagenomics is the study of microbial communities in their original habitats, which can give a comprehensive insight into the interactions within these communities. Metagenomics can also help identify individual species within microbial habitats. Shotgun metagenomics refers to the approach of shearing DNA extracted from environmental samples and sequencing the small fragments to study not only the microbial species composition, but also the gene functions and metabolic pathways within them. 16S/18S/ITS Amplicon-based sequencing is a DNA sequencing method that focuses on sequencing specific target regions, amplicons, to understand the species composition and diversity within the community.

Target Capture Sequencing (TCS) allows researchers to extract genome information from exons or regions of interest in the human or mouse genome with a wide selection of panels. TCS technologies, such as Whole Exome Sequencing (WES), provides a higher coverage of rare variant identification than whole genome sequencing and can deliver exceptional prospects for researchers.

Centyle Biotech provides a fully flexible genotyping service for small, large, standard or customized projects, on humans and many other species. Our powerful portfolio includes arrays, reagents, instruments and bioinformatics tools that enable you to detect common and rare single nucleotide polymorphisms (SNPs), copy number variations (CNVs) and other genetic variations. CD Genomics genotyping services accommodate projects with a broad range of applications like ddRAD, GWAS, GBS, Skim Seq, Low Pass Whole Genome Sequencing

Amplicon sequencing involves sequencing specific lengths of PCR products or captured fragments to analyze variations within the sequences. This technique allows for high-coverage sequencing of target regions tailored to different requirements and can detect low-frequency mutations. Currently, amplicon sequencing primarily encompasses functional gene sequencing and targeted sequence-directed capture fragment sequencing.

Amplicon sequencing is based on NGS technology or PacBio SMRT sequencing. The ultra-deep sequencing of amplicons (PCR products) allows efficient variant identification and characterization. This technique has a wide range of applications, including 16S/18S/ITS gene sequencing, SNP genotyping, CRISPR sequencing, somatic/complex variant discovery, antibody screening sequencing, immune repertoire sequencing

Viral genome sequencing is a vital technique in modern virology, utilizing advanced high-throughput sequencing technologies. It involves acquiring and analyzing viral genome sequences using bioinformatics, with Illumina's Next-Generation Sequencing (NGS) being a popular choice for fragment analysis. Recent advancements, such as PacBio's Single-M Real-Time (SMRT) sequencing, offer longer reads and reduced errors, addressing limitations of traditional methods. Centyle genomics platform promises to enhance viral genome sequencing, aiding in the interpretation of viral metagenomics data and improving our understanding of viruses.

Mitochondrial DNA (mtDNA) sequencing is a powerful tool for studying genetic variations and understanding evolutionary relationships. This technique focuses on analyzing the DNA in mitochondria, the energy-producing organelles in cells. By examining mtDNA, researchers can gain insights into maternal lineage, population genetics, and the genetic basis of certain diseases. It is widely used in fields such as anthropology, forensics, and medical research to uncover valuable information about genetic heritage and health

Chloroplast DNA sequencing is a powerful technique used to analyze the genetic material found within chloroplasts, the organelles responsible for photosynthesis in plants. This method provides insights into plant evolution, phylogenetics, and biodiversity by revealing the structure and function of chloroplast genomes. Researchers utilize chloroplast DNA sequencing to investigate species relationships and enhance our understanding of plant biology and conservation efforts. It is an essential tool for botanists, ecologists, and geneticists alike.

Shallow Whole Genome Sequencing (shallow WGS) is an innovative and cost technology that enables accurate genome-wide genetic variation analysis across a diverse array of species including humans, livestock, and plants. Utilizing shotgun sequencing, shallow WGS achieves low coverage (typically between 0.4x and 1x) while still delivering over 99% accurate variant calls. This method offers enhanced data output, improved statistical power, and the ability to discover rare variants, making it a valuable tool for genome-wide association studies, evolutionary analysis, and molecular breeding. Additionally, shallow WGS can help build customized reference panels tailored to specific populations.

ChIP-Seq is a powerful technique that enables the specific enrichment of DNA fragments associated with target proteins through chromatin immunoprecipitation. This process involves purifying and constructing libraries enriched DNA fragments, which are then analyzed using high-throughput sequencing. Researchers can map millions of sequence tags to the genome, revealing interactions between DNA and histones, transcription factors, and other proteins. Key applications of ChIP-Seq include studying gene regulation, transcription complex assembly, DNA repair, histone modification, developmental mechanisms, and disease progression.

ATAC-Seq, or Assay for Transposase-Accessible Chromatin using sequencing, is a powerful technique used to study chromatin accessibility and identify regulatory regions in the. By utilizing a transposase enzyme, ATAC-Seq allows researchers to analyze open chromatin regions, providing insights into gene regulation and cellular function. This method is widely used in various fields, including epigenetics and developmental biology, to enhance our understanding of expression dynamics.